Features of the cytogram and cytokine profile of bronchoalveolar lavage fluid in experimental metabolic syndrome

The aim of the study was to identify features cellular composition and cytokine profile bronchoalveolar lavage fluid in rats a model diet-induced metabolic syndrome. Materials methods. In an experiment on animals (rats), syndrome (MS) induced by high-fat high-carbohydrate diet reproduced. To assess viability reproduced model, biochemical morphometric methods were used, such as measurement body weight, specific gravity liver visceral fat, blood pressure, determination glucose concentration (including tolerance test), well lipid parameters. intensity inflammatory response blood, total protein, number leukocytes, levels immunocytokines (interleukin (IL)-6, IL-10, tumor necrosis factor (TNF)α, monocyte chemoattractant protein (MCP)-1) determined. Open performed isolated heart – lung complex. (IL-6, TNFα, MCP-1), ratio their morphological types determined (BALF). Results. with MS, increase leukocytes due granulocytes rise IL-10 revealed compared parameters controls. BALF analysis absolute alveolar macrophages, neutrophil granulocytes, lymphocytes. IL-6 MCP-1 more than 1.5 times higher. Conclusion. Changes qualitative quantitative are nature formed during systemic accompanying disorders modeling MS experiment.